FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8815957
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139640.15839460197004138No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT90090.10218969988170315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.050.2878841
CAACGCA146100.049.910355
TCAACGC146650.049.7634434
GTATCAA153300.049.3475651
ATCAACG149000.049.01853
AACGCAG157850.046.356666
GTCTCGC20700.037.062961
TCTGTCG21150.035.976228
ACGCAGA212650.034.717427
TATCAAC215200.034.436872
CGCAGAG216800.034.0273748
GCAGAGT239400.030.8656359
AGAGTAC187500.028.94587310-11
GAATACG39200.027.6002859
AGTACGG198950.026.07170912-13
TCTCGCT30050.025.1366372
CAGAGTA203100.024.86397710-11
GAGTACG196700.024.54141812-13
CTCGCTC30200.024.0270713
CTGTCGC36850.023.3915379
Produced by FastQC (version 0.11.5)