FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8826010
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT683100.7739624133668555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328960.3727165502871626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279840.31706286306043163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103070.11677983596211651No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT98580.1116925994871975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112800.077.655611
GTATCAA341600.065.1520161
ATCAACG399100.055.49693
TCAACGC401050.055.2839134
CAACGCA407350.054.339715
AACGCAG421400.052.52296
TATCAAC448250.049.6108742
ACGCAGA514600.043.1139647
CGCAGAG521100.042.5987748
TCTGTCG22150.042.4400868
GCAGAGT557450.039.8743679
GTCTCGC24350.039.3460351
AGAGTAC509300.032.11396810-11
GAATACG38650.030.3256179
CAGAGTA523900.029.48715210-11
CTCGCTC32750.028.8908233
TCTCGCT33800.028.3454462
CTGTCGC41550.028.0658349
GTGCTAG49800.027.4835361
AGTACTT428450.026.4329512-13
Produced by FastQC (version 0.11.5)