FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14205199
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG383690.270105332561691No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC298650.21023992694505722No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC199440.14039930028435366No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG151600.1067214897869435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA61000.050.6880344
GCGACAT66450.046.7437255
CTGCGAC79600.039.0228463
CGACATC88750.035.0519266
GCTGCGA88950.034.9240232
CGCACTA119100.031.5430537
TAGCCCA58700.029.0657791
GTCCGCA131000.028.787474
TAGCCCC50400.028.7174911
GCACTAA141350.026.9138438
TCCGCAC143800.026.4223615
CACTAAG144050.026.2774989
TCGACCG22800.026.0393093
CCGCACT146550.026.0236826
GTGTCCG145700.025.8853152
TGTCCGC150450.025.1605643
GCGCGGT57900.024.5262766
TAGGCCC46900.024.4227851
ACATCTG134850.023.104258
GGTCGAC142500.023.03035716-17
Produced by FastQC (version 0.11.5)