FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14205199
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286410.2016233633896998No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG148690.1046729440397139No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA147170.10360291327140154No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG142420.10025906712042541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC65350.044.650388
TCCGACC65800.044.5728389
TTTCCGA82800.035.6338547
TCACCCC233050.035.1444329
TTCACCC239700.034.041548
CGGGGAT118950.033.011158
CGTTTCC81150.032.9172335
CCGTTTC86850.032.8151634
CCGATCG95850.032.36016586-87
ACCCGAT97800.031.81027484-85
TCGCGCG60750.031.499137
ACCGGTT15300.031.3988343
ATAGCGC87700.031.395894-95
GATCGGC100050.031.21879888-89
TCCGTTT83750.031.0989063
CCGTTTA29100.031.0811614
CTCCGTT71050.030.6876412
GTTTCCG85650.030.6440016
CGATCGG95950.030.31070186-87
TCGGGGA132700.029.8705967
Produced by FastQC (version 0.11.5)