FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17363596
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG4313492.484214675347203No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA2071811.1931917789379574No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2038371.173933095425625No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2014701.1603011265638754No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1846351.0633454037976926No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1364220.7856782661840324No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1323640.7623075312279783No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1059450.6101558686345846No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG978430.5634950271821575No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA946520.5451174975506226No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG833760.48017703245341575No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA770230.44358898928539914No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA756640.43576226952066843No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA702520.40459361067834104No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG670320.38604906495175306No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG628090.3617280660066037No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG549130.3162536147466228No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG547770.31547036685258056No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG517400.29797975027753465No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA509880.2936488501575365No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT508610.29291743484471766No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA483490.2784503855077024No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA454610.2618178861106881No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC454140.2615472048531882No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC451570.26006709670047606No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA451360.2599461540109549No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA433870.2498733557265442No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA413330.23804400885623No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA402260.23166860136575396No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA393880.22684241213628792No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG388910.22398010181761888No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA387990.2234502576540021No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG386190.22241360602953444No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC349930.20153083497220278No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG347090.1998952290758205No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT346890.19978004556199075No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG343340.19773553819151285No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA334760.19279416544821706No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT329560.18979939408864385No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG328880.18940777014162272No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCT314510.181131834672956No Hit
GACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTT308280.17754386821715962No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA298240.17176165582290673No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC297360.17125484836205587No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG294430.1695674098844502No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA288030.16588153744189854No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT283480.163261112502272No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA276380.15917209776131624No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC276210.15907419177456097No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT272220.1567762806736577No Hit
GGCGCGATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG264530.15234747456690423No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA263050.15149511656456416No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC260970.15029720802073487No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA260340.1499343799521712No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA258000.14858673284036325No Hit
GCCCTGTCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG249390.143628082569993No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT247050.14228043545818503No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG241260.13894587273281409No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA240130.13829508587967607No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA239590.13798409039233578No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG237650.13686681030818731No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA228080.1313552791714343No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG226310.13033590507404114No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC225130.12965632234244567No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA222510.12814741831127607No Hit
GCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTG217430.12522175706000072No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG214570.12357463281223544No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA212620.12245159355239547No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG212510.12238824261978913No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC211750.12195054526723613No Hit
CAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGA211570.12184688010478935No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG207760.11965263416633283No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG207460.11947985889558822No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA206800.11909975329995007No Hit
TAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT206640.1190076064888863No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG204880.1179939915671846No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG202800.11679608302335528No Hit
TCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTA200600.11552906437122816No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA197590.1137955524880906No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA195110.11236727691660185No Hit
AGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGG194770.1121714649430913No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC193710.11156099231979366No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG193060.111186645899847No Hit
GGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTA189130.10892328985309264No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG187320.1078808790529335No Hit
GGGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG181380.10445992869219024No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT178920.10304317147208446No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA178450.1027724902145846No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA174660.10058976262751103No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT174200.10032484054570262No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)