FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23912483
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC3122711.3058911531688282No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC1415940.5921342421863928No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC322370.13481243248557667No Hit
GGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTATCCAACATCAACATC313230.1309901610803027No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT289720.12115847609802796No Hit
CAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGA285240.11928497764117595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA172100.081.650144
GCGACAT177000.079.389765
CTGCGAC189550.074.333853
GCTGCGA200200.070.332072
CGACATC206750.067.897186
GGCTGCG241300.059.1697121
CTAGGCG570500.057.5944337
TAGGCGG579350.056.7256748
GCTAGGC593350.055.4484946
GGCTAGG611550.054.1943555
TGGCTAG622450.053.2987374
AGGCGGG674750.048.7969789
CTGGCTA692350.048.1851733
GGCTGGC716150.047.323031
GACATCT301750.046.804387
GCTGGCT725750.046.177022
ACATCTG310950.045.314968
ACCCGAC52550.044.3712433
CATCTGT344950.040.8209539
CGGTCGA419300.038.2617768-69
Produced by FastQC (version 0.11.5)