FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17308687
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2492041.4397625885776315No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1975701.141449955158355No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1157170.6685486888751295No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1077470.6225024463149631No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC816380.47165911544879174No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC718950.4153694615888542No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG646880.3737314101294916No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG592080.3420710074657887TruSeq Adapter, Index 7 (100% over 49bp)
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA521070.3010453652550306No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG488060.28197401686216866No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC480830.27779692359102687No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG480650.27769292956767894No Hit
CAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAA394410.2278682374925377No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT372300.21509430495796705No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT333520.19268937037222986No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC331690.1916320978015259No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA312900.1807762772531504No Hit
ACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCA311610.18003098675249024No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT308590.17828619813854166No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG260160.1503060284122071No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC230280.13304302053645087No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTC225410.13022940446031522No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA225290.1301600751114166No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC223180.12894103405994922No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC217160.12546301172353513No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG216150.12487948970363841No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC213730.12348134783418292No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC211160.12199654427860415No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC195130.11273529875489689No Hit
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC189130.10926883130996591No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG185530.10718895084300734No Hit
CAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGG178620.10319673583559516No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA175890.10161949314815157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)