FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17308687
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG817890.47253150975576597No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC714190.41261939741587567No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC527280.3046331590605342Illumina Single End PCR Primer 1 (100% over 50bp)
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG488900.282459322304459No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG455260.26302399482987937No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA433440.2504176082218137No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC429070.24789286443275566No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC390300.22549370729276No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG373280.2156604946406391No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA324900.18770921214301234No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG286820.16570869875918376No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG262760.15180816430501054No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA236880.13685613472587493No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG235500.1360588472135408No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG234100.13525000480972357No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222220.12838639926876025No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA219060.12656072641442992No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC192800.11138915389711536No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG190400.11000256691914297No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA190120.10984079843837952No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA182370.105363277988677No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG181100.10462954237949995No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG177820.10273454017627101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTA91150.056.7195325
GGTGTTA118000.054.4150542
TGCTAGT61250.052.8823624
CAGTACG309150.052.5732088
TGATCGT51450.051.768125
GTGCTAG62050.050.5140463
TTCAGTA251700.049.977436
TTCGACG8200.049.3027461
TCAGTAC332250.048.978927
AGTAGCA935150.045.801338
GTAGCAA945400.045.4181379
GTACACC78200.045.230727
TAGTAGC945150.045.139077
CTGATCG60200.044.7187584
CGTCGGT127550.043.980749
TTAGTAG149100.043.523356
TGATAGT103000.043.4532434
TCGACGT10700.042.6731572
GGCGCGT11950.042.5874751
TACGCAT28250.042.4276852
Produced by FastQC (version 0.11.5)