FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13419704
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC402530.2999544550311989No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG259760.193566117404676No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG212700.1584982798428341No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG149840.11165671016290674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA66150.062.5794034
GCGACAT72900.056.7201965
CTGCGAC79750.051.9668123
GCTGCGA85050.048.8934862
CGACATC116400.036.1727836
GGCTGCG124300.033.9902271
TAGCCCA57000.032.2885441
ACATCTG132400.031.5159118
TAGCCCC49850.031.0353221
CATCTGT149150.028.2617329
TAGGCCC45350.028.0639441
GGTCGAC171700.026.47490916-17
AAGCTTG192200.026.3817463
TGATCGC79500.026.33093524-25
TAGCCCG13300.026.3241141
TTGATCG80150.026.14687522-23
CAAGCTT195500.025.5488192
TTGGGCT203400.025.0219947
GACATCT172350.024.6493577
TCGACCC185700.024.27538518-19
Produced by FastQC (version 0.11.5)