FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13419704
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG183890.13702984805029977No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164500.12258094515348475No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA158460.1180801007235331No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA152460.11360906321033609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA210000.045.4944089
TTCCGAC61050.043.5510068
TAGTAGC228300.041.728977
AGTAGCA236750.040.3616188
TCCGACC66100.039.9507039
CAGTACG69650.037.1583188
TTTCCGA73400.036.477877
TCACCCC205650.036.1839649
CGGTGAA88350.035.576665
GTACGGT15950.035.435842
AGTACGA73550.035.3911139
GCTAGTA24750.034.081895
CCGTTTC70600.033.9032754
TCGCGCG59900.033.5243237
CGACCTA9100.033.1196861
CGAGAAC186500.033.04570824-25
CGTTTCC69150.032.9836735
AAACGAG187300.032.9728322-23
CAAACGA188050.032.7629320-21
ACGAGAA188900.032.7260524-25
Produced by FastQC (version 0.11.5)