FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16741653
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG444170.2653083300675268No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA272430.16272586703355996No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG231980.13856457304425077No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG47300.054.8313145
TCCGACC75550.047.8456159
TTCCGAC78000.046.3427778
CTGTCGG55350.045.826934
CGGGGAT278000.042.2714238
GTCGGGG64250.041.9186336
TCGGGGA288350.040.8699387
TCTGTCG62350.040.6054233
TTTCCGA98450.037.247647
GTTTCCG84950.036.9043666
CCCCGTT47850.036.7219852
CCGTTTC96350.034.9535834
TCACCCC259350.033.534679
CGTTTCC91500.033.3800545
ATCGGGG212000.033.160656
ATAGCGC141350.032.69205594-95
TCTATCG160650.032.169293
CTATCGG169250.031.9101584
TATCGGG179750.031.8695225
CCGATCG156150.031.61378586-87
Produced by FastQC (version 0.11.5)