FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21544967
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC759960.35273203249742735No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT438200.20338856866199886No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT343520.15944327043991297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT104700.074.301175
TGCGACA105200.073.406124
CTGCGAC114000.067.9939043
GCTGCGA122650.063.39222
CGACATC124250.062.495666
GGCTGCG150300.052.4803771
ACTCGGG226200.035.6696431
ATTCGGC109300.035.43992238-39
TTGATCG115800.033.68671422-23
TTCGGCT115350.033.6836140-41
TGATTCG116450.033.40668536-37
GACATCT237250.033.0898747
TGATCGC117700.033.0218524-25
CTCGGGA245200.032.948722
TCGGCTG119550.032.53997840-41
ACATCTG242650.032.3341378
TCGGGAG252450.031.9836643
TCGCCAG129200.030.59177826-27
GGTGTGC196450.030.5337267
GATCGCC130900.029.85525724-25
Produced by FastQC (version 0.11.5)