FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646050
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC890120.37643496482499195No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC586240.24792301462612149No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT448230.18955808686863135No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT385840.1631731303959858No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC359190.1519027490849423No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA259550.10976463299367126No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT258060.10913450660892622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA90500.064.9733054
GCGACAT94150.062.3628925
CTGCGAC103450.057.161013
CGACATC113150.051.8916
GCTGCGA115350.051.4287452
GGCTGCG150950.040.1694071
ACTCGGG270850.034.3176771
CTCGGGA289800.032.221532
TCGGGAG304000.030.8414383
CTAGGCG317850.030.070037
TAGGCGG330100.028.8965688
TGGCTAG340650.028.415724
GCTAGGC341200.028.1096486
GGCTAGG356350.027.1811985
GCGCGGT80000.026.3645956
ACATCTG233600.025.6634258
GACATCT237450.025.1272857
GGCTGGC413100.024.2374631
TTGATCG122350.024.16604622-23
CTGGCTA404550.024.0798913
Produced by FastQC (version 0.11.5)