FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23646050
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG408970.1729548909860209No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG301830.12764499779032862No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA241590.10216928408761716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC135800.053.2711459
TTCCGAC141850.051.1330348
TTTCCGA172200.042.452377
TGTCGGG60900.042.1185955
CGGGGAT273600.041.1977548
GTTTCCG157450.039.9431886
TCGGGGA288650.039.2640767
TCACCCC488200.038.655629
CGTTTCC155300.038.5998235
CCGTTTC159700.038.428484
CTGTCGG65300.037.31644
GTTCCGT37950.036.7941471
TTCACCC526900.035.8164258
GCTCCGT112100.035.5467221
GTCGGGG77200.035.1946076
TCCGTTT163850.035.1643453
GTTCACC539050.034.9478237
CTCCGTT130600.034.326882
ATAGCGC298800.033.84089394-95
GTCCCGT37700.033.3847161
Produced by FastQC (version 0.11.5)