FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18544176
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1276970.6886097284667704No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC881750.4754862119513965No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC461380.24880048593153992No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA354590.19121367269163106No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT242680.13086588479315553No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT194540.10490625196827295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA123650.070.2933654
GCGACAT127600.068.162295
CTGCGAC136800.064.091873
GCTGCGA144850.060.5299842
CGACATC155850.055.8374066
GGCTGCG174400.051.0012321
ACATCTG240450.036.547158
GACATCT246700.035.6212467
TGGCTAG407400.033.6925744
CTAGGCG412450.032.892247
CATCTGT266650.032.8670929
GCTAGGC424550.032.066676
GGCTAGG431400.031.7226665
TAGGCGG427700.031.6750148
ACCCGAC40800.031.43123
CTGGCTA454700.030.4593013
GGCTGGC453850.030.297551
GCTGGCT480200.028.5055352
ACTCGGG171700.028.3368131
GCGCGGT63900.027.7277416
Produced by FastQC (version 0.11.5)