FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18696730
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG331510.1773090802509316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG40550.044.3845255
CGGGGAT221350.040.5278058
TCCGACC51100.040.5197079
TCGGGGA227350.039.8764347
TTCCGAC54800.038.0438658
CTGTCGG51400.034.091534
ATCGGGG163600.033.4400066
CCCCGTT52500.033.10182
GTCGGGG58100.032.6132436
CCCCGAA46600.032.5038342
CCCGTTA23550.031.4546183
TATCGGG136600.031.3573325
TCTATCG124950.031.2762223
CCGATCG82750.029.9243786-87
CCCCCGT46550.029.8861241
TCTGTCG59700.029.5087173
CTATCGG137950.029.2948384
CCGTTTC84650.029.171564
ATAGCGC79850.029.02327594-95
GTCCCGT28150.029.01161
Produced by FastQC (version 0.11.5)