FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20928701
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG663030.3168041819700133No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA439640.21006559365533486No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG359610.17182623995631646No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG346140.16539010232885454No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA319280.15255605209324744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC152250.052.7238249
TGTCGGG72400.052.4848635
TTCCGAC159400.050.3588568
CTGTCGG79800.046.3081324
TCTGTCG80000.045.716943
GTCGGGG89650.044.293366
CGGGGAT405150.043.63538
TCACCCC536300.043.309049
TTTCCGA193000.041.9612777
TCGGGGA425600.041.684127
CGTTTCC161850.041.3798645
GTTTCCG172050.040.5278936
CCGTTTC164850.040.2808654
GTTCACC590550.039.322857
TTCACCC594400.038.9958578
GTTCCGT42050.038.401371
GTCGGTT36950.036.63593
TCCGTTT173600.036.5538523
ACCGGTT26400.036.3432583
ATAGCGC356650.035.121494-95
Produced by FastQC (version 0.11.5)