FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21652034
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC374150.056.8630376
GTGTGCT530900.054.5610127
GTGCTCT532650.053.883559
CGGTGTG137850.052.494975
TGTGCTC552100.051.985288
CCGATCT282400.051.39218516-17
TCCGATC273750.050.57388316-17
CCGGTGT82700.047.094634
CGATCTA63100.042.67589618-19
TGTCGGG61300.040.7684975
GGGTGTG264000.040.7086525
CTTCCGA468200.039.5745514-15
CGGGGAT283200.039.4040958
GCGGTGT44950.038.588414
CGGGTGT73400.038.2842064
CTGTCGG63400.037.825914
TCTGTCG63250.037.7706383
TCGGGGA300300.037.343147
TCCGACC83400.036.152829
CGATCTG110650.035.41400518-19
Produced by FastQC (version 0.11.5)