FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17354496
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1193530.6877353280671475No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC498480.28723392485728194No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC359970.207421753993893No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA273850.1577977257305542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA81200.061.301524
GCGACAT84500.059.0389675
CTGCGAC93300.053.6059233
GCTGCGA100050.049.894382
CGACATC100800.049.114916
TCGACCG23000.045.4318963
CTAGGCG324100.039.9771927
GGCTGCG127350.039.669171
TGGCTAG332750.039.382174
GCTAGGC339100.038.292886
TAGGCGG338000.038.2909858
GCTCGCG28100.037.5369579
GGCTAGG349750.037.5208055
CCGCTCG29050.036.4724467
GGCTGGC368650.035.4388281
CTGGCTA378900.034.873743
CTCGACC31100.033.7518772
GCTGGCT398000.032.79442
CGACCGC32500.032.736384
AGGCGGG422200.030.745039
Produced by FastQC (version 0.11.5)