FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201367
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164210.1345832807094484No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG160970.13192784054442425No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA129840.10641430587244856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC43750.043.9725049
TTCCGAC48600.039.6690988
TCGCGCG58800.037.913967
GTAGCAA89000.036.608949
CGGGGAT125000.036.3146258
TAGTAGC96800.034.2290887
TCACCCC162450.034.1920179
TCGGGGA135600.033.645817
CGCGCGT68350.032.6892628
CCTCGCG63000.032.3088265
CCGTTAC10800.031.8455354
TTTCCGA62500.031.6726637
CCGTTTC62850.031.559224
GCGCGTC72300.030.9780759
ACCCGAT54600.030.52424684-85
CCGTTTA19150.030.2611734
ACGAGAA68950.029.87869624-25
TCTATCG67800.029.87123
CTCCGTT52050.029.8556772
CGAGAAC67600.029.7056324-25
Produced by FastQC (version 0.11.5)