FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12782795
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC427600.33451213134529656No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC196220.1535032048937654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT65500.066.084935
TGCGACA66850.064.6796954
CTGCGAC75450.057.4952133
GCTGCGA81100.053.1975172
CGACATC87750.049.8668756
GGCTGCG107000.040.987761
ACATCTG134300.032.6527868
GACATCT135750.032.304017
TTGATCG73350.029.6014722-23
CATCTGT150700.029.2561139
TGATCGC76900.028.48135424-25
TAGCCCC45750.027.4039381
GATCGCC81100.026.86069724-25
GGTCGAC130450.026.5333116-17
TAGCCCA66000.026.2358631
TAGGCCC40450.025.3804571
GTGTGCT127200.025.1135358
TCGACCC138100.024.97796418-19
GGTGTGC90100.024.1257767
TTCGGCT90800.023.80879440-41
Produced by FastQC (version 0.11.5)