FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12782795
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA181450.14194861139523868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC48800.039.6098758
GTAGCAA159600.039.090579
TAGTAGC161550.038.5199747
TCCGACC52200.036.6775749
AGTAGCA179600.034.8340538
TCACCCC173950.034.72729
AGTACGA46050.034.7148329
TTTCCGA58750.033.8628547
CAGTACG48400.033.508318
TCGCGCG50150.032.9955377
TGTCGGG25300.032.966865
CGGGGAT103550.032.5988588
TCGGGGA107200.032.059137
ATAGCGC78700.031.82705794-95
GCCCCGT36500.031.6098521
CGAGAAC130650.030.96340824-25
CCGTTTC58950.030.9318664
CGAAGTG129500.030.70745740-41
ACCCGAT86800.030.69037484-85
AAACGAG131600.030.66821522-23
Produced by FastQC (version 0.11.5)