FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14985170
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2047451.3663174992342428No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1108810.7399382189191047No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG820710.5476814744177076No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA778540.5195403188619149No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC630540.4207760072124641No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA618900.4130083275665207No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC599120.3998086107798577No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT392410.26186556442135794No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT346910.23150221185345246No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC288600.19259040771642896No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT281050.18755209316944688No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT272440.18180641260659705No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT254070.169547626086324No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG252380.1684198444195161No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG238190.15895048237690995No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT236820.15803624516772247No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT233210.15562719675519196No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG226390.15107603050215648No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC223490.1491407838549713No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA222450.14844676436770488No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG215380.1437287665071534No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT197680.13191708869502314No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC196030.1308160000854178No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC190060.12683206129793656No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG188850.1260245963175593No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT188190.12558416087371715No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC184100.12285479577475598No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC171200.11424628482693223No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG168620.11252458263736748No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT167410.1117171176569902No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT163760.10928137618725713No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC162060.10814692125614858No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG161730.1079267035342275No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG159570.10648527844528957No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC154920.10338221054549265No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA152440.10172724099893429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC210700.056.7866136
GCGACAT20850.054.4470255
TGCGACA21250.053.4216044
CCGACTC225550.053.0057145
ACTCCCT226850.052.869468
CTGCGAC21950.051.9343
TCAGACG33400.051.0540585
ACCCGAC246550.048.4515883
CCCGACT248500.048.262854
TAGAGCC135150.047.9380767
AACCCGA258250.046.4243472
AGACGTG37100.046.3465047
CTCCCTT262200.045.868379
GACTCCC261750.045.8020557
TTAGAGC142300.045.2289736
TATTCGT182350.044.4395872
GCTGCGA26850.043.166052
GAACCCG280550.042.6337781
ATTCGTA193850.041.8256573
CTCACGT527150.040.2768639
Produced by FastQC (version 0.11.5)