FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17805391
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG867900.4874366420821649No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT805920.45262696000329344No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG552400.3102431168178222No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC467680.26266202185618953No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA417310.2343728368559837No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA391230.2197255876043385No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC351900.19763677191924628No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT295050.1657082397123433No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC281740.15823297561957497No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT199610.11210649628531046No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG198350.11139884543956378No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT197210.11075858991245965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCC156000.054.9889346
CCGACTC163800.052.3124125
TGCGACA27800.049.2070584
ACCCGAC177100.048.383683
CCCGACT181450.047.5377434
GCGACAT28800.047.003965
ACTCCCT192900.044.469938
AACCCGA194350.044.212712
CTGCGAC31100.044.138583
GACTCCC204500.041.9475447
TATTCGT106350.039.2594762
GAACCCG219900.039.2068181
TAGAGCC138050.038.5013357
GCTGCGA36750.038.1292082
CTCCCTT239300.036.1846929
ATTCGTA115850.035.998083
TTCGTAC117850.035.4273764
TTAGAGC151850.034.8772476
TCGATCG130350.033.3235916-17
ACGCATT75450.032.798913
Produced by FastQC (version 0.11.5)