FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17158797
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3187551.8576768522874887No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1835691.0698244171779643No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1193630.6956373456717274No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1054550.6145827122962059No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1025850.5978565979887751No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC982630.5726683519829509No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT856010.49887529994089913No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA819210.47742857497527363No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT814760.4748351530704629No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT638540.3721356456399595No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG500150.29148313835754336No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC448960.2616500445806311No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT435600.25386395095180625No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT425820.24816425067561554No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT418900.2441313339157751No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC408630.23814606583433556No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT402490.23456772639713613No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT391640.22824443928091231No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT390780.22774323864312865No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC377390.21993966127112524No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC370840.21612237734381962No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC364970.21270139159522664No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG354720.2067277793425728No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC352420.2053873590322212No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG352390.2053698752890427No Hit
CCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAAC351990.2051367587133294No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG350640.2043499902702969No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG346870.20215286654419887No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG343710.20031124559606364No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA329960.19229786330591825No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC323690.18864376098161195No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC316970.18472740250962816No Hit
GAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTG314600.1833461867985267No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC313330.18260604167063693No Hit
GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCC300310.17501809713116834No Hit
GGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCT298220.17380006302306625No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC294730.17176612089996754No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT269900.15729540946256315No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA264620.1542182706631473No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC258410.15059913582519802No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC256160.14928785508681056No Hit
CGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCA254180.14813392803702963No Hit
CCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTAC251320.1464671445206794No Hit
CCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC247920.14448565362711616No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT246500.1436580897833339No Hit
ACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACA240700.14027789943549074No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG236430.13778937998975102No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA230520.1343450825835867No Hit
GGGTTGTTTCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCC228310.13305711350277064No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG226320.13189735853859685No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT225500.13141946955838454No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC220760.12865703813618168No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG219100.1276896043469714No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC218970.12761384145986457No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG213860.1246357772051269No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA208380.12144208011785441No Hit
GGCTACCTTGTTTCGACTTCACCCCAATCATCTGTCCCACCTTCGGCGGC198570.11572489609848523No Hit
CCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCG197480.11508965342966641No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTCGGCGGC194540.11337624659817351No Hit
AGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGT193680.11287504596038987No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG189040.11017089368211537No Hit
ACACGTGGAATTCCACTCTCCTCTTCTGCACTCAAGTTCCCCAGTTTCCA183030.10666831713202272No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG179710.10473344955360216No Hit
ACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAG179220.10444788174835333No Hit
CAGACCTATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAAT178230.10387091822346287No Hit
CCTTGCCGAAACAGTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTAC175710.10240228379646894No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA174620.10176704112765014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)