FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16608915
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC505740.30449911990036677No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC359450.216419916653195No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC339650.20449860812702092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA71500.072.414134
GCGACAT72100.071.482115
CTGCGAC80950.064.136643
GCTGCGA87250.059.7238732
CGACATC96750.053.366466
GGCTGCG113300.046.412221
GACATCT154200.033.8222547
TTGATCG83850.030.73141322-23
ATTCGGC86500.030.25759738-39
TGATCGC85400.030.1456524-25
TGATTCG87200.030.1237436-37
ACATCTG174000.029.863878
GGTGTGC156450.029.1157237
TTCGGCT91150.028.81823740-41
TCGGCTG93400.027.97143640-41
GATCGCC95850.027.13159224-25
TCGCCAG96350.027.01544226-27
GTGTGCT231100.026.5957578
ATCGCCA104750.024.91706726-27
CATCTGT208750.024.8470139
Produced by FastQC (version 0.11.5)