FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16608915
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341040.20533550806900994No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA195890.11794268319152697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCT229000.045.1822369
GTGTGCT240950.043.178597
GGTGTGC192900.042.6945956
TGTGCTC257850.039.9981548
CGGTGTG66900.038.36475
CCGATCT150800.032.20172516-17
TCCGATC148750.031.38562616-17
TTCCGAT162000.030.99717114-15
CTTCCGA170850.028.65569514-15
TGTCGGG35100.027.9784625
CGGGGAT157350.027.7978848
GTCCCGT24400.026.8335821
GGGTGTG174650.026.7021085
TCGGGGA163950.026.6215367
TCTTCCG197050.026.4464412-13
CCGATCG53900.025.48333286-87
GCGGTGT30350.025.1670174
ATAGCGC50550.024.87345994-95
ACCCGAT55300.024.66603584-85
CGGTCGA26750.024.12412638-39
Produced by FastQC (version 0.11.5)