FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19778467
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454240.22966390671228465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCT229600.037.711017
GGTGTGC186200.037.221696
GTGCTCT244950.034.574599
CGGTGTG64800.032.912435
TGTGCTC262000.032.306148
GTACGGT20050.030.0870882
CCGATCT120850.029.95213116-17
TCCGATC119900.029.71402516-17
CTTCCGA142050.026.8688914-15
TTCCGAT147050.026.4882914-15
TAGTAGC135950.025.2587877
CGAGAAC89650.025.2216124-25
ATCGCGA28800.024.9065618-19
TCAGTCG81250.024.90016784-85
CGCGAGG72700.024.75742166-67
ACGAGAA97300.024.26384524-25
GTAGCAA144600.024.2093039
AAACGAG98350.024.14966822-23
CAAACGA94900.024.10247220-21
TCTTCCG174100.023.9958212-13
Produced by FastQC (version 0.11.5)