FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134479
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC406190.30925474851343554No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC389450.2965096674180986No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC247160.18817647810773463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA58150.070.167714
GCGACAT59950.068.060925
CTGCGAC69000.059.6159743
GCTGCGA72600.056.7381972
CGACATC82650.049.4245576
GGCTGCG99350.041.7194441
GACATCT130200.031.8117667
ACATCTG141550.029.2274238
GGTGTGC107650.029.0331467
TTGATCG70900.029.00885222-23
TGATCGC73350.028.04108624-25
TGGCTAG181850.026.2249244
CTAGGCG176550.026.1236467
TAGGCGG178550.025.9374358
ATTCGGC79100.025.46116438-39
TCGCCAG82250.025.38224426-27
TGATTCG79850.025.2517636-37
GCTAGGC184850.025.130826
CATCTGT164350.025.114579
GTGTGCT155650.024.9624088
Produced by FastQC (version 0.11.5)