FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089825
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG375870.24908837577639237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT22350.043.9930345
TGCGACA22650.042.5714954
CTGCGAC27650.035.2164463
GCTGCGA30000.033.414452
GGTAGTA130850.033.179025
TAGTAGC134350.032.4558687
GTAGTAG144650.030.4731796
GTAGCAA146500.029.8285879
ATACCGG31950.028.2465389
GGTGTGC110850.028.024017
AGTAGCA163100.026.8803278
TGGTAGT162800.026.550834
GTGGTAG179300.025.0344643
AATACCG36500.024.985918
CGACATC39750.024.257566
CGCTTCG29300.024.23612842-43
CTAGGCG58550.023.932247
GTCGGTC67650.023.76686586-87
TAGGCGG59300.023.5493778
TCAAACG97650.023.42144820-21
Produced by FastQC (version 0.11.5)