FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190908
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC97200.11866816230874525No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC92440.11285684078004539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT18500.055.2010085
TGCGACA19200.053.4358634
CTGCGAC22500.046.020813
GCTGCGA25700.040.110182
CGACATC26400.039.222286
GGCTGCG36650.028.7878041
GGTGTGC59950.028.1267437
CGAAGCG13650.023.31522848-49
GTGTGCT90150.022.655858
TTGATCG23500.022.43609822-23
TGATCGC24850.020.83538824-25
CTAGGCG48000.020.4837467
TAGGCGG48950.020.1831198
TGATTCG26350.019.46839136-37
TGTGCTC71500.019.397659
TCGCCAG27850.019.18796226-27
CGCTTCG16350.018.88390442-43
GATCGCC28300.018.6310824-25
ATTCGGC27900.018.30163438-39
GACATCT59800.017.9509547
Produced by FastQC (version 0.11.5)