FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12767706
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG286720.22456657444963096No Hit
AACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGA205100.16063966385190886No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC163020.12768151146337486No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC151070.1183219601077907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT31250.053.0477335
TGCGACA31850.052.346684
CTGCGAC42200.040.5204933
CGACATC43650.038.1955346
GCTGCGA44400.038.0918122
GGTAGTA102450.032.037425
TAGTAGC111950.029.3186327
GTAGTAG115600.028.762726
GTAGCAA116350.028.4546179
GGCTGCG63300.027.8550031
ATACCGG26050.027.7154679
GGTGTGC91400.027.1277667
AATACCG27050.027.042278
TGGTAGT128150.026.0201594
CGCTTCG40800.025.90316442-43
AGTAGCA132350.024.7636648
CGAAGCG43150.024.382748-49
GTGGTAG143100.023.7329143
GTAATAT91850.023.478298
TGATCGC37250.022.50695224-25
Produced by FastQC (version 0.11.5)