FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13996190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299420.21392964799706207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC36800.039.7091839
TTCCGAC39550.036.348318
CCGATCG57200.029.86938386-87
CTCCGTT45850.029.8020322
TTTCCGA49450.028.7840797
ATAGCGC55700.028.20473594-95
TCGATCG15050.027.7417766
ACCCGAT62600.027.36831784-85
CGGTGTG31950.026.5812195
TCCGTTT60700.026.419363
CATAGCG65900.025.78673794-95
GTTTCCG45700.025.7468176
GTTCCGT17900.025.711381
CGGCATA62250.025.46490390-91
CGGGGAT90700.024.8470278
CCGTTTC62100.024.8306224
TCACCCC134850.024.6987089
ATTGCGC22100.024.688449
GCTCCGT44150.024.6099931
ATCGGCA65550.024.39805288-89
Produced by FastQC (version 0.11.5)