FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18539825
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC285650.15407373046940842No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC275600.14865296732844027No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC258060.13919225235405405No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG202190.10905712432560719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT48900.052.842455
TGCGACA49500.052.4898154
CTGCGAC57650.046.0580333
CGACATC58650.044.0565956
GCTGCGA64150.041.096242
GGCTGCG87200.031.2684171
ACGGACC38800.029.2589744
GGTGTGC133900.025.2923377
CGGACCA47350.024.1763085
CACGGAC48450.023.5293733
GCGCGGT43450.023.1753396
GGTCGAC44900.023.11503216-17
ATACCGG44000.022.8854649
GGTAGTA101700.022.232025
AACACGG56150.021.9109151
GTGTGCT205950.021.7022298
ACTCGGG137050.021.6279831
CTAGGCG162700.021.5451157
AATACCG46700.021.460618
TTGATCG62550.020.95877322-23
Produced by FastQC (version 0.11.5)