FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12166748
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC326740.2685516294082856No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC299920.2465079411523934No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC239300.1966836166903432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA45650.066.3838354
GCGACAT48700.062.1287965
CTGCGAC55200.055.3291973
CGACATC58350.051.7722366
GCTGCGA59200.051.7518462
GGCTGCG80000.039.2465131
GACATCT115100.026.6997917
CTAGGCG154250.025.5274987
TTGATCG59050.025.49902522-23
GGTGTGC97800.025.4976047
TGGCTAG162100.025.0826384
TGATCGC59950.025.03766324-25
TAGGCGG159000.024.5855468
ACATCTG127300.024.0289388
GCTAGGC165100.023.9362956
GTGTGCT150700.023.292088
GGCTAGG172600.023.116445
GATCGCC66300.022.99785824-25
ATTCGGC66350.022.6591638-39
TGATTCG67350.022.49895336-37
Produced by FastQC (version 0.11.5)