FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14453117
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC285880.1977981635380105No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC247070.17094582435055358No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC244580.1692230125861432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA49750.063.3008164
GCGACAT51050.061.4097145
CTGCGAC60850.052.222113
GCTGCGA64950.049.4383432
CGACATC64850.048.2673876
GGCTGCG97550.033.452521
TTGATCG61200.025.92242622-23
GGTGTGC118100.025.3778487
ATTCGGC64150.024.6957738-39
GACATCT129850.024.471447
TGATTCG64600.024.37646336-37
TGATCGC64400.024.2291624-25
TTCGGCT66250.023.84125940-41
GTGTGCT185800.023.3651378
ACATCTG144200.022.0687318
TCGGCTG72750.021.87435340-41
CTAGGCG142950.021.5975537
TCGCCAG75400.021.19837626-27
GATCGCC75200.021.0336824-25
TAGGCGG146600.020.9298638
Produced by FastQC (version 0.11.5)