FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14453117
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451380.3123063350279389No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA163680.11324892754967664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG59150.029.4482186-87
CGGGGAT113000.029.0114388
ACCCGAT62750.027.98582384-85
ATAGCGC58750.027.06408794-95
TCGGGGA122050.026.8222057
TCCGACC32150.026.26689
GATCGGC67750.025.92130388-89
CCCTACG52000.025.8316822-23
CGATCGG63000.025.19681286-87
TGTCGGG30550.024.5372585
TAGTGCG74800.024.36369974-75
GCCGAAC77900.024.18382566-67
CATAGCG74400.023.63926194-95
TCACCCC129150.023.5100579
TTCCGAC36800.023.4635228
ATCGGCA67650.023.39527388-89
TATCGGG71700.023.3580045
TACGTTC56650.023.2079824-25
GTCCCGT24050.023.0820031
CCTACGT58350.022.61379622-23
Produced by FastQC (version 0.11.5)