FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14381833
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC264970.1842393803348989No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG206570.14363259537223105No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC188740.13123501016873163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT49600.065.661955
TGCGACA50150.064.567524
CGACATC53650.062.548216
CTGCGAC58000.056.2332043
GCTGCGA62900.051.4100882
GGCTGCG88150.037.4284861
GGTAGTA83850.031.2296495
TAGTAGC86850.030.2384437
GTAGTAG91400.029.28576
TTGATCG57450.028.588622-23
GTAGCAA95600.027.9391169
GACATCT122500.027.8516487
ATTCGGC58450.027.73830438-39
GGTGTGC98050.027.1277737
TGATCGC60150.026.9932624-25
TGATTCG60700.026.74876636-37
TTCGGCT61650.026.2223640-41
ACATCTG135700.025.017958
GTGTGCT154350.025.0125528
TCGCCAG68750.024.29921226-27
Produced by FastQC (version 0.11.5)