FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14381833
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253530.17628490054084203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC27350.031.924089
CCGATCG48100.029.68074286-87
ACCCGAT51100.027.93813384-85
TTCCGAC31900.027.6681528
TCGATCG23350.027.4344166
ATAGCGC48650.027.05280794-95
GTTCCGT18850.026.1796151
CCCGTTA16350.026.1193873
TAGTGCG60600.025.8320574-75
CCCCGTT38750.025.3477782
ACCGACT45750.025.1003157
GCCCCGT27500.024.8468341
CGGCATA53300.024.82698490-91
CGGGGAT109800.024.676968
CATAGCG58450.024.34600494-95
GCCGAAC65650.024.24269566-67
GTCCCGT22450.024.0951581
ATCGGCA56800.024.09117988-89
CGACTGA48500.023.6772639
TCGGGGA117700.023.4236477
Produced by FastQC (version 0.11.5)