FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16039653
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC346480.2160146481971898No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC275060.17148750038420407No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC269790.16820189314569337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA66400.064.0330054
GCGACAT69400.061.7383585
CGACATC72900.058.7932786
CTGCGAC83300.051.379943
GCTGCGA86400.049.4867552
GGCTGCG115000.037.9135251
GACATCT135950.031.8566367
GGTGTGC110800.030.2270957
TGATCGC75700.028.05068424-25
TTGATCG76200.027.86662322-23
GTGTGCT172950.027.6356418
ACATCTG167450.026.1982388
GATCGCC85800.024.83071924-25
TGATTCG87950.024.33058236-37
ATTCGGC88750.024.16413738-39
TTCGGCT91450.023.57891840-41
CATCTGT190000.023.058889
TCGCCAG96150.022.52387826-27
GATTCGG95300.022.47870438-39
TCGGCTG97100.022.08610240-41
Produced by FastQC (version 0.11.5)