FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14360921
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC321020.22353719514228926No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC226080.15742722907534967No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC183800.12798622038238355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA56700.068.4457864
GCGACAT57700.067.503535
CGACATC64850.060.320246
CTGCGAC68850.056.23083
GCTGCGA72500.053.6672
GGCTGCG105550.037.6182331
TTGATCG66600.029.48688122-23
GACATCT136900.029.0658537
ACATCTG136850.028.9120718
GGTGTGC106550.028.8120487
TGATCGC68650.028.46969824-25
ATTCGGC69400.028.12856138-39
GTGTGCT160100.027.8332588
TGATTCG71450.027.42002936-37
TTCGGCT73350.026.8374840-41
TCGGCTG77550.025.20246940-41
CATCTGT162600.024.574749
GATCGCC80450.024.41056324-25
TCGCCAG82600.024.28651426-27
TGTGCTC130600.021.7438669
Produced by FastQC (version 0.11.5)