FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14360921
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505780.35219189632754055No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA149590.10416462843852424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC31950.034.1585439
TTCCGAC37200.029.8480018
CCGATCG64400.029.51027786-87
ATAGCGC61350.029.12086394-95
ACCCGAT68450.027.79835984-85
CGGGGAT118800.027.519798
CCGTTAA14550.027.3941084
CCCGTTA16050.026.902843
TGTCGGG31000.025.8679085
GATCGGC75050.025.64066188-89
TCGGGGA129900.025.3872997
CGGCATA71250.025.37486890-91
CGATCGG71100.025.12565686-87
TAGTGCG81950.024.72670274-75
GTCCCGT19450.024.6401941
CATAGCG76800.024.6236794-95
ATCGGCA73800.024.4975888-89
CCCTACG54100.023.87252422-23
GCCGAAC86100.023.86613866-67
TCACCCC143750.023.8326159
Produced by FastQC (version 0.11.5)