FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14221601
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC400350.2815083899485016No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC270740.19037237790597558No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC205010.14415395284961235No Hit
AACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGA151710.10667575331356856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA70850.070.2790154
GCGACAT71300.070.098755
CGACATC76300.064.771676
CTGCGAC82700.060.4357953
GCTGCGA87050.057.749572
GGCTGCG116750.044.022181
GACATCT135600.037.025177
ACATCTG147800.033.740678
TTGATCG78000.032.12693822-23
TGATCGC79000.031.75002524-25
TGATTCG88650.028.4266636-37
GATCGCC88300.028.32630724-25
ATTCGGC89450.028.06750138-39
CATCTGT184650.027.1276459
TTCGGCT93500.026.9267940-41
GGTGTGC105000.026.3385167
TCGGCTG97450.025.88350740-41
TCGCCAG98600.025.79565626-27
CGCTTCG39600.025.41887542-43
GATTCGG99500.025.37403338-39
Produced by FastQC (version 0.11.5)