FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17273534
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC1104350.6393306662087793No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC174010.10073792658757612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATC146300.088.141986
TGCGACA155700.082.795114
GCGACAT157150.082.001315
CTGCGAC166600.077.518993
GCTGCGA169800.075.900612
GGCTGCG196500.065.896951
GACATCT221600.058.495087
ACATCTG244400.053.2665868
CATCTGT282850.045.9058239
TTGATCG158200.040.4894622-23
TGATCGC162250.039.34863324-25
TGATTCG165400.038.89726336-37
ATTCGGC165850.038.8907538-39
TTCGGCT167000.038.4261940-41
GATCGCC170150.037.5768524-25
TCGGCTG173500.037.1759240-41
GATTCGG176050.036.5975138-39
TCGCCAG176650.036.3535626-27
GGTGTGC155950.036.198437
ATCGCCA181100.035.3048126-27
Produced by FastQC (version 0.11.5)