FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17273534
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559560.32394065973992353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC200550.048.3868566
GTGTGCT261400.047.7432487
CGGTGTG66350.047.344975
TGTGCTC270200.045.328048
GTGCTCT273350.045.1528939
TCCGACC46450.038.9227529
CCGGTGT43000.038.7342764
TTCCGAC52750.034.6339958
CCGATCT183850.033.3685616-17
TTCCGAT187800.033.1255114-15
TGTCGGG39300.032.600785
TCCGATC178250.032.59285716-17
CGGGGAT175900.032.1839188
TCGGGGA188400.030.3506477
CTTCCGA199300.030.02363814-15
ATAGCGC90550.029.85716294-95
CCGATCG99900.029.38997886-87
TCACCCC191700.029.0362289
TCTTCCG222000.028.99503112-13
GCGGTGT30800.028.8102914
Produced by FastQC (version 0.11.5)