FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15472225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT23350.045.0170255
TGCGACA23450.044.424834
CTGCGAC26350.039.5355723
CGACATC27650.037.148746
GCTGCGA32750.032.3859252
GGTGTGC98700.028.3773297
CGCTTCG23150.026.9591742-43
CGAAGCG25300.023.92627148-49
GTGTGCT156300.023.8088078
GGCTGCG52900.020.8482691
GGTAGTA86450.020.8440425
GTCGGTC33900.020.34827886-87
TAGTAGC84750.020.1336277
TCGAAGC32350.019.72742846-47
CTAGGCG55900.019.680337
TGTGCTC123250.019.6733469
TAGGCGG55900.019.2784678
TTGATCG27750.019.27736722-23
TCCGATC22500.019.18730416-17
TCGGTCC32550.019.17415888-89
Produced by FastQC (version 0.11.5)