FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11986801
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335620.2799913004311993No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA129270.10784361899392507No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG126440.10548268883416018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC27350.038.3460779
TTCCGAC31050.033.929498
CGGGGAT115700.031.992438
TGTCGGG25900.031.880465
TCGGGGA120450.031.1639167
CCGATCG54250.031.04268386-87
ATAGCGC51800.030.40566894-95
ACCCGAT57000.029.502684-85
GATCGGC61500.027.69452988-89
TATCGGG72200.027.5392385
CTGTCGG28400.027.4031584
CGATCGG58550.026.89672386-87
ATCGGGG87600.026.8690746
CGGCATA59400.026.79460590-91
TCACCCC115100.026.5519569
ATCGGCA61450.026.09346888-89
CCCGTTA14400.026.033823
TCTATCG66000.026.0278283
TCTGTCG30300.025.9979293
CCGTTTC48000.025.7043864
Produced by FastQC (version 0.11.5)