FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15458632
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA27200.046.928314
GCGACAT29000.044.3391655
CGACATC34600.036.658116
GGTGTGC121300.035.7185867
CTGCGAC39750.033.174483
GCTGCGA42400.030.9942042
GTGTGCT186650.030.1573968
TGTGCTC151600.027.4302589
CGCTTCG29950.022.79682742-43
CGAAGCG29950.021.8567548-49
GGCTGCG66550.020.7338121
TCCGATC36300.019.77848616-17
TGATCGC34050.019.01823624-25
TTGATCG35050.018.67645322-23
ATTCGGC35150.018.22293738-39
TCGCTTC37900.017.70537242-43
TGATTCG37150.017.49451836-37
TTCGCTT39850.017.31003640-41
TCGAAGC40250.017.13798146-47
CTTCGCT42100.016.32918440-41
Produced by FastQC (version 0.11.5)