FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15458632
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356180.23040848633954156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT21800.029.1675641
CCGATCG22350.027.52849686-87
CCGTTAT15200.025.5991154
CCCGTTA16800.024.0079963
CCCCGAA33200.023.7257022
CCGTTTA29850.023.2093644
CGATCGG24100.023.16386686-87
CCCGTAA21750.022.9074273
CCCGTTT53000.022.8302463
GGTCCGT23550.022.567271
GTCCGTT20800.021.6725162
CCGTTAA16800.021.4663894
CCCCGTA20650.021.1405772
ACCCGAT29550.020.98158884-85
GATCGGC30100.020.67799488-89
GCCCGTT28350.020.4200062
GCCCCGT30050.020.0544661
TCCGTTA18300.019.9657633
ATAGCGC28350.019.85978594-95
TAGTGCG32300.019.6361874-75
Produced by FastQC (version 0.11.5)