FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004913278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451686
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT16850.37304676257400055No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG16750.37083283519967414No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC13660.30242247933298794No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG13520.299322981008931No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC11730.25969368100848816No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA9090.2012459983262709No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT8320.18419875754395754No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG8300.18375597206909225No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC7740.17135797877286435No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC7440.1647161966498851No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG7250.1605097346386649No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA6910.1529823815659551No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC6740.1492187050296002No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG6160.136377926258507No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT6140.13593514078364174No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG6140.13593514078364174No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG5810.12862918044836458No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG5660.12530828938687497No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG5440.12043764916335685No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT5340.11822372178903044No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT5270.11667397262700194No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG5250.11623118715213666No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG4770.10560433575536989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)